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Cleaning of Genomic DNA

  1. Dissolve pellet 200uL TE pH 8.0, add 0.5 uL RNAase, digest 37C for 1hr
  2. Add 0.5 volume 7.5M ammonium acetate + 2.5 volume EtOH (precipate) -> spin @4000g, pipette off liquid (don't disturb pellet) and dry. Re-elute in TE.
  3. Add 0.10 volume 3M Na Acetate and 2 x volume EtOH (precipitate), spin, wash with 70% EtOH and dry
  4. Final elution in H20 or TE pH 8.0.
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