Cleaning of Genomic DNA
- Dissolve pellet 200uL TE pH 8.0, add 0.5 uL RNAase, digest 37C for 1hr
- Add 0.5 volume 7.5M ammonium acetate + 2.5 volume EtOH (precipate) -> spin @4000g, pipette off liquid (don't disturb pellet) and dry. Re-elute in TE.
- Add 0.10 volume 3M Na Acetate and 2 x volume EtOH (precipitate), spin, wash with 70% EtOH and dry
- Final elution in H20 or TE pH 8.0.