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Plasmids

Vectors

Vector ID

Construct

Host Vector

Stock

Selection

notes

D1

35S: SpeI,XbaI,BamHI,SacII :nos

pUC19

DH5

amp

see pdf

D2

35S: purpurea F3'H :nos

pUC19

DH5

amp

D3

35S: quamoclit F3'H :nos

pUC19

DH5

amp

D4

35S: purpurea F3'H :nos

Cambia 1301

Top10

kan

D5

35S: quamoclit F3'H :nos

Cambia 1301

Top10

kan

D6

35S: GUS :nos

pUC18

Top10

amp

D7

35S: purpurea DFR-B :nos

pUC19

Top10

amp

D8

35S: purpurea DFR-B :nos

Cambia 1301

Top10

kan

D9

35S: purpurea DFR-B :nos (alt cut sites)

pUC19

Top10

amp

D10

35S: coccinea F3'H :nos

pUC19

Top10

amp

D11

35S:purpurea DFR-B:nos:35S:purpF3'H:nos

pUC19

DH5

amp

D12

35S:purpurea DFR-B:nos:35S:purpF3'H:nos

Cambia 1301

DH5

kan

D13

35S: quamoclit ANS :nos

pUC19

Top10

amp

D14

35S: quamoclit ANS :nos

pCB302

Top10

kan

D15

His tag:: Solanum DFR

pDEST17

DH5

amp

D16

His tag:: coccinea DFR-B

pDEST17

DH5

amp

D17

unused

 

 

 

D18

35S: BglII,XhoI :nos

pUC19

Top10

amp

see pdf

D19

quamoclit F3'H promoter:GUS:nos

pUC18

Top10

amp

D20

purpurea F3'H promoter:GUS:nos

pUC18

Top10

amp

V21

35S: quamoclit DFR-B :nos

pUC19

Top10

amp

V22

35S: quamoclit DFR-B :nos

Cambia 1300

Top10

kan

V23

35S: coccinea ANS :nos

pUC19

Top10

amp

V24

35S: coccinea ANS :nos

Cambia 1300

Top10

kan

V25

35S: coccinea F3'H :nos

Cambia 1301

Top10

kan

V26

35S:GFP:nos

pUC19

Top10

amp

V27

35S: IpMYB1 :nos

pUC19

Top10

amp

Q299

Petunia DFR-A promoter: GUS :nos

pUC18

Top10

amp

Quattrocchio

35S:Luc

35S: Luciferase :nos

pUC18

?

amp

Sun Lab

35S:Ren

35S: Renilla :nos

pUC18

?

amp

Sun Lab

Host Plasmids

Host plasmids

Use

selection

Stock

Source

pUC19

all purpose cloning vector

amp

Top10

 

pDEST17

bacterial overexpression vector

amp

DB3.1

Invitrogen

Cambia

binary vectors- complete 2004 collection

various

DH5

Cambia

pGSA1165

binary; source of the weak MAS promoter

cam; plant-kan

DH5

ABRC

pDONR221

GATEWAY entry vector

Kan

DB3.1

Benfey lab

pCB302

binary vector; poor MCS

kan; plant- BASTA

 

Dong Lab

pCB302-3

binary vector; targets cytosol

kan; plant- BASTA

 

Dong Lab

pH7GWIWG2(I)

binary; double stranded RNAi; GATEWAY

spec; plant-Hyg

 

 

pHGWFS7

binary; GUS::GFP; GATEWAY

spec; plant-Hyg

 

 

Bacterial Stocks

Bacterial Stocks

Use

Top10

General cloning

DH5

Library storage

DB3.1

Library storage; resistant to ccdB effects

GV3101

Agrobacterium strain; helper Ti plasmid contains rif resistance

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