Duke Wiki  logo
Child pages
  • Sequencing Reaction
Skip to end of metadata
Go to start of metadata

Direct Sequencing

Setup on Ice
10uL reactions
Make one Master Mix for forward primer and a second for reverse primer

Reagent

Amount

Big Dye

0.5

Buffer

1.5

Primer

1

Water

6.5

Template

0.5

Total

10


Step

Temp

Time

denature

96C

30s X 25

anneal

50

15s

extension

60

4min

end

4

infinity

  • No labels