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pGEM-T easy PCR cloning

vector (in freezer in little styrofoam box):

per 4 ul ligation reaction:

2X ligation buffer: 2.0 ul

PCR product: 1.2 ul
pGEM-T easy vector: 0.4 ul
T-4 DNA ligase: 0.4 ul (use only Promega ligase in that same box)

Incubate at room temp for 2-4 hours, or at 4C overnight.

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